Chromatography of Milk Proteins on DEAE Cellulose,

Separation of vitamin B 12-binding proteins of serum, gastric juice and saliva by rapid DEAE cellulose chromatography.

By FRANCOIS P. RETIEF, CHESTER W. GOTFLIEB, SHAUL KOCHWA, PETER W. PRATF AND VICTOR HERBERT N ORMAL SERUM contains at least two binders of vitamin B12, one migrating electrophoretically as an alpha-globulin2’3 and the other as a beta-globulin.46 There is evidence that these substances have different metabolic functions. Whereas native B12 is bound to alpha-globulin,2 the betaglobulin binder pro...

Separation of folate binding protein from human serum by DEAE-cellulose column chromatography.

On diethylaminoethyl-cellulose column chromatography, the folate binding protein in the serum of 21 patients eluted in the early effluents as a single sharply defined peak. The chromatographic behavior of the folate binder remained unchanged whether or not the serum was, before chromatography, complexed with tritium-labeled pteroylglutamic acid ([3H]PGA), dialyzed, or charcoal-adsorbed. Heating...

Purification of peptidyl-tRNA on benzoylated DEAE-cellulose columns.

The o-nitrophenylsulfenyl group, and amino protecting group in the chemical synthesis of peptidyl-tRNA was used to attach newly synthesized peptidyl-tRNA to a benzoylated DEAE-cellulose column. This facilitated the isolation of a highly purified specific tRNA with a well defined peptide chain.

Electrophoresis on cellulose acetate and chromatography on DEAE-sephadex A-50 compared in the estimation of creatine kinase isoenzymes.

We have compared two methods for separating the isoenzymes of creatine kinase in serum and measuring their activity: chromatography on diethylaminoethyl-Sephadex A-50, with both continuous and discontinuous gradient elution, and electrophoresis on cellulose acetate. Results by continuous and discontinuous gradient elution correlated well both for tissue extracts and sera. Electrophoresis on cel...

Gel electrophoretic analysis of peanut proteins and enzymes. I. Characterization of DEAE-cellulose separated fractions.